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However, by now, ATR FT-IR spectroscopy as succeeded many other sampling techniques, since it is mostly non-destructive, very easy to apply and suited to analyze solids and liquids in their present states. Diffuse reflectance infrared fourier transform spectroscopy (DRIFTS) is a special reflection sampling technique that allows to collect great quality spectra of solid samples that are very difficult to analyze in transmission, like soil or concrete.
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Here the IR light only interacts with the surface of a material to collect chemical information. Gaussian deconvolution of FTIR spectrum of collagen (a w 0.38) in the (OH) region, by means of four component bands, as obtained by means of second derivative method. If a sample is thin enough (<15 µm), as for example a polymeric film, a coating on a metallic surface or a biological tissue section, enough IR light can pass the sample to be analyzed directly without dilution in KBr or a solvent.Īnother technique would be reflection. Classically, an otherwise solid sample is either ground with IR transparent potassium bromide (KBr) and pressed into a pellet, or it is thinly sliced and placed onto a KBr window, while liquids are directly measured or diluted with an IR transparent solvent, e.g. The secondary structures of OPI have been quantified by the deconvolution of the amide I band of the FTIR spectrum of OPI, which were found to contain approximately 7 beta-turn, 19 alpha-helix, and 74 beta-sheet. Deconvolution was performed based on the studies of Duer et al.
#DECONVOLUTION WITH ESSENTIAL FTIR SOFTWARE#
Deconvolution of the peak was carried out using OriginPro 2017 software (Multiple Peak Fit) and Gaussian functions. This depends on the sample that needs to be analyzed. The amino acid contents of OPI are 4.5-8.7 times higher than those of oat flour. The spectra were processed using TopSpin 3.5 and OriginPro 2017 software.